Figure EV2. PC1 and PC2 are autophagy substrates that are degraded in the lysosome.

• A
  MEFs, Saos2 and RCS were untreated or treated with 100 nM BafA1 for 6 h in MEFs, 100 nM BafA1 for 9 h in Saos2, 200 nM BafA1 for 6 h in RCS, then lysed and analysed by Western blot. Bands were visualized with antibodies against PC1, PC2, LAMP1, SQSTM1/p62, LC3 and β‐actin. Western blots are representative of three independent experiments.
• B, C
  (B) MEFs or (C) RCS treated with vehicle, 100 nM BafA1 for 4 h, followed by 4‐h washout. Cells immunolabelled with LAMP1 (488, green) and PC1 (568, red). Nuclei were stained with Hoechst (blue). Scale bars = 10 μm.

Source data are available online for this figure.