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. 2018 Dec 17;38(2):e99847. doi: 10.15252/embj.201899847

Figure 3. FAM134B is required for autophagy recognition of PC1.

Figure 3

  1. Bar graph shows quantification of lysosomes (LAMP1+) containing PC1 expressed as % of total number of lysosomes (mean ± SEM) in Saos2 cells mock transfected or transfected with siRNA against the indicated genes and treated with 100 nM BafA1 for 9 h. n = 20 cells per condition; three independent experiments. One‐way ANOVA with Dunnett's multiple comparisons test performed, ***P < 0.0001.
  2. MEF cell lines lacking the expression of indicated genes were treated for 12 h with 50 nM BafA1, fixed and immunolabelled for PC1 (568, red) and LAMP1 (488, green). Scale bar = 10 μm. Insets show magnification of the boxed area. Bar graph (on the left) shows quantification of LAMP1 vesicles positive for PC1, expressed as % of total lysosomes (mean ± SEM), n = 12, 10, 12, 10, 7, 10 cells per genotype, respectively; three independent experiments. One‐way ANOVA with Dunnett's multiple comparisons test performed and P‐value adjusted for multiple comparisons. ns ≥ 0.05, ***P < 0.0001.