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. 2018 Dec 17;38(2):e99847. doi: 10.15252/embj.201899847

Figure 4. PC1 accumulates intracellularly in cells lacking Fam134b.

Figure 4

  1. WT and CRISPR‐Cas9 Fam134b knockout MEFs were treated as indicated, lysed and analysed by Western blot with the indicated antibodies. Western blots are representative of 4 independent experiments.
  2. WT and CRISPR‐Cas9 Fam134b MEFs were immunolabelled for PC1 (568, red), nuclei stained with Hoechst (blue) and analysed by scanning confocal microscopy. Scale bar = 10 μm.
  3. CRISPR Fam134b MEF mock, wild‐type FAM134B‐HA or FAM134Blir‐HA transfected were immunolabelled for PC1 (568, red), Lamp1 (488, green) and HA (647, violet) and analysed by scanning confocal microscopy. Scale bar = 10 μm. Inset panels show magnification of the boxed area. Bar graph shows quantification of Lamp1 vesicles positive for PC1, expressed as % of total lysosomes (mean ± SEM), quantification of n = 10 cells per treatment; three independent experiments. One‐way ANOVA with Dunnett's multiple comparisons test was performed. ns ≥ 0.05, ***P < 0.0001.

Source data are available online for this figure.