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. 2018 Dec 17;38(2):e99847. doi: 10.15252/embj.201899847

Figure 7. CANX and FAM134B interact and deliver PC to autophagosomes.

Figure 7

  1. Schematic representation of FAM134B WT, lir mutant and delta reticulon constructs.
  2. U2OS cells were transfected with empty vector control (pcDNA3), FAM134B‐HA WT or mutant constructs as indicated.
  3. U2OS cells were transfected with HALO‐PC2, FAM134B‐HA or FAM134Blir‐HA constructs, treated with 100 nM BafA1 for 6 h and with CST where indicated. (B, C) Complexes were immune‐isolated with HA‐magnetic beads, separated by Western blot and visualized with antibodies against HALO, CANX, FAM134B, LC3 and β‐actin. 5% of the input is shown. Western blots are representative of three independent experiments. Dashed line indicates that unnecessary lanes were removed.
  4. Proposed model of collagen recognition by autophagy. After synthesis, PC chains are subjected to quality control operated by the CANX/CRT chaperone system. Properly folded PC associates with HSP47 and is then secreted, whereas the misfolded fraction is sequestered by the CANX‐FAM143B complex and delivered to lysosomes through ER‐phagy.

Source data are available online for this figure.