Domain architecture of human Parkin with positions of selected PD‐associated mutations. Ubl, ubiquitin‐like; IBR, in‐between‐RING (Gladkova
et al,
2018). HeLa cells expressing wild‐type Parkin or the indicated PD‐associated Parkin mutants were treated with antimycin A and oligomycin (
AO) for 2 h. Immunoblots are representative of three independent experiments. Graph shows densitometric analysis of non‐ubiquitinated proteins in AO‐treated samples relative to untreated control from three independent experiments. Error bars represent mean ± SD. All Parkin constructs were N‐terminally FLAG‐tagged except R104W.