Skip to main content
. 2015 Sep 9;20(9):16290–16305. doi: 10.3390/molecules200916290

Figure 2.

Figure 2

Interaction of Xanthorrhizol and tadA protein. Tryptophan quenching assay (A–C) were used to observe interaction between xanthorrhizol and tadA protein; (A) wild type tadA; (B) tadA (A143V); and (C) tadA (F149G). The assays showed a response, as revealed by fluorescence signal quenching, that xanthorrhizol bound to tadA protein. Tryptophan quenching assay demonstrated that binding capacity of xanthorrhizol to tadA mutants were lower than wild type, as shown by calculated Kd 19.04 μM, 109.7 μM, and 32.78 μM for wild type, A143V, and F149G, respectively.