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. 2015 Aug 28;20(9):15748–15765. doi: 10.3390/molecules200915748

Table 2.

Genotoxicity of B. carinata and sinigrin in SMART. Frequencies of mutations (spots/wing) for each category (Small, Large, Twin and Total) obtained in simple treatment.

Genotoxicity
Mutation Rate (Spots per Wing) Diagnosis a
Treatment N° of wings Small single spots 1–2 cells; m = 2 Large single spots > 2 cells; m = 5 Twin spots m = 5 Total spots m = 2
Controls
H2O 212 0.26 (54) 0.04 (8) 0.03 (5) 0.32 (67)
H2O2 (0.12 M) 168 0.60 (94) + 0.07 (11) − 0.06 (4) − 0.65 (109) +
Plant Material: Brassica carinata (mg·mL−1)
1.25 40 0.01 (4) − 0.03 (1) − 0.03 (1) − 0.15 (6) −
2.50 48 0.15 (7) − 0.04 (2) − 0.02 (1) − 0.21 (10) −
5.00 48 0.13 (6) − 0.02 (1) − 0.02 (1) − 0.19 (8) −
Single Compound: Sinigrin (mM) b
0.60 40 0.18 (7) − 0.03 (1) − 0 0.20 (8) −
1.20 34 0.12 (4) − 0.03 (1) − 0.03 (1) − 0.18 (6) −
2.40 36 0.08 (3) − 0.03 (1) − 0 0.11 (4) −
4.81 28 0.14 (4) − 0 0 0.14 (4) −

a Statistical diagnoses [33,34]: + (positive) and − (negative). Significance levels α = β = 0.05, one-sided test without Bonferroni correction; b 1 mM sinigrin = 397.46 µg·mL−1 sinigrin.