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. 2015 Jul 21;20(7):13264–13280. doi: 10.3390/molecules200713264

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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The effect of 16 on the viability of human leukemic cells (HL-60). Cell viability (viable cells-black bar; apoptotic cells-gray bar; and necrotic cells-white bar) was determined respectively by fluorescence microscopy using acridine orange/ethidium bromide after 24-h incubations. The data are presented as the mean values ± S.E.M. of three independent experiments performed in duplicate. The negative control was treated with the same vehicle (NC, 0.1% DMSO) that diluted the test substance. Doxorubicin (PC, 0.3 μg/mL) served as the positive control. * p < 0.05 compared to negative control by ANOVA, followed by a Student Newman-Keuls test.