Table 6.
The transitions and optimized MS parameters of 13 target markers and internal standard in the UPLC-QqQ-MS/MS analysis.
| Compounds | Rt (min) | Precursor Ion (m/z) | Daughter Ion (m/z) | Con Voltage (V) | Collision Energy (eV) |
|---|---|---|---|---|---|
| Taurine | 0.82 | 126.00 | 108.20 | 22 | 14 |
| Ginsenoside Rg1 | 1.25 | 823.30 | 643.50 | 80 | 40 |
| Arenobufagin | 2.23 | 417.20 | 399.10 | 45 | 30 |
| TDCA | 3.39 | 522.28 | 486.61 | 50 | 25 |
| TCA | 3.41 | 538.00 | 538.00 | 60 | 2 |
| Astragaloside | 4.91 | 807.00 | 807.00 | 80 | 30 |
| Bufalin | 5.22 | 387.30 | 351.20 | 35 | 22 |
| CA | 5.41 | 391.30 | 355.30 | 0 | 20 |
| Cinobufagin | 5.56 | 443.50 | 365.20 | 60 | 20 |
| Resibufogenin | 5.59 | 385.30 | 367.20 | 35 | 18 |
| Ginsenoside Rg3 | 5.75 | 807.00 | 365.00 | 90 | 5 |
| CDCA | 6.13 | 357.60 | 161.20 | 40 | 20 |
| DCA | 6.23 | 357.60 | 161.20 | 40 | 20 |
| Tanshinone IIA | 7.28 | 295.10 | 277.00 | 40 | 20 |