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. 2015 Aug 26;20(9):15449–15468. doi: 10.3390/molecules200915449

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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IC₅₀ values of different plant extracts on surface displayed Hyal-1. (A) Equiseti herba; (B) Malvae sylvestris flos; (C) Ononidis radix. Activity of surface displayed Hyal-1 was measured after incubation with extract of each plant at concentrations ranging from 0 to 10 mg/mL. The IC₅₀ value was calculated from the inflection point (50% inhibition of the Hyal-1 activity) of the plot. The different shape of the curves probably results from the heterogeneous composition of the extracts and from the different solubility of the constituents in the extracts (n = 3, error bars ± SD).