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. 2015 Nov 12;20(11):20297–20311. doi: 10.3390/molecules201119693

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Derinat protected skin cells from UVB damage. The effect of UVB irradiation on cell viability in (A) keratinocytes (KCs) and (B) human dermal fibroblasts (HDF) (***, p < 0.001). The cell survival probability for cells pretreated with different concentrations of Derinat for 24 h and irradiated with or without UVB-exposure in (C) KCs and (D) HDF (*, p < 0.05; ***, p < 0.001). Further confirming the result of (C,D), in (E) KCs and (F) HDF were pretreated with 15 μg and 150 μg of Derinat and irradiated with 50 mJ/cm² and 100 mJ/cm² UVB, respectively. The cell survival probability was analyzed using an MTT assay after 24 h of UVB-exposure (*, p <0.05; ***, p <0.001).