The effect of Derinat on the intracellular Ca2+ concentration of UVB-exposed skin cells. (A) Experimental design for the treatment of each group: control, UVB, EDTA, UVB+D and Derinat only; (B) Ca2+ calibration curve; (C,D), Mean value of intracellular Ca2+ for control, UVB-exposed, UVB-exposed with different concentrations of EDTA, UVB-exposure with Derinat and Derinat in KCs and HDF cultured in BSS for 30 min (*, p < 0.05; ***, p < 0.001); (E) KCs and (F) HDF were pretreated with or without the Derinat for 24 h and exposed to UVB. The intracellular Ca2+ concentration was then measured after 24 h of incubation in normal medium (***, p < 0.001).