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. 2015 Sep 2;20(9):16030–16047. doi: 10.3390/molecules200916030

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect on HCV translation of the aptamers P6-86, P6-96, P6-103, and P7-49 in Huh-7 cells. (A) Schematic diagram showing the genetic organization of the reporter dicistronic replicon Rep-FLuc used in this study; (B) Huh-7 cells were co-transfected by electroporation with the RNA aptamers and 5.2 μg of a mixture containing the transcripts Rep-FLuc and cap-RLuc. HCV IRES activity was measured as the activity of FLuc protein and referred to that obtained for RLuc. Luciferase activity in the control reactions with the non-related RNA80 is established as 100%. Data points are the mean of three independent experiments.