Table 3.
Epoxycarvone stereoisomers effect on pilocarpine-induced seizures test.
| Experimental Group | Latency to Convulsions (s) | Latency to Death (s) | Peripheral Cholinergic Signs (%) | Stereotyped Movements (%) | Tremors (%) | Seizures (%) | Status Epilepticus (%) | Mortality (%) |
|---|---|---|---|---|---|---|---|---|
| Control (Tween 80 5%) | 429.3 ± 25.4 | 817.6 ± 22.3 | 100 | 100 | 100 | 100 | 100 | 100 |
| Diazepam (standard) | 3600.0 ± 0.0 b | 3600.0 ± 0.0 b | 100 | 0 | 50 | 12.5 | 0 | 0 |
| (+)-cis-EC (7) | 862.6 ± 36.6 b | 1845.9 ± 453.1 | 100 | 50 | 100 | 100 | 0 | 75 |
| (−)-cis-EC (8) | 890.8 ± 76.2 b | 1135.9 ± 68.1 | 100 | 100 | 100 | 100 | 50 | 100 |
| (+)-trans-EC (9) | 1044.0 ± 49.1 b | 2895.7 ± 343.3 b | 50 | 100 | 50 | 100 | 50 | 50 |
| (−)-trans-EC (10) | 888.4 ± 78.3 b | 2345.0 ± 477.9 a | 50 | 100 | 50 | 100 | 50 | 50 |
The values represent the mean ± SEM (n = 8 in each group). a p < 0.05; b p < 0.001 for the epoxycarvone stereoisomers group (300 mg/kg) as compared to the control group (ANOVA One way/Tukey’s test). Fisher’s exact test was used to analyze the percentages of animals with peripheral cholinergic signs, stereotyped movements, tremors, seizures, status epilepticus and the mortality rate.