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Primers design of MIP-LAMP. (A), location and orientation of L. monocytogenes specific MIP-LAMP primers within the nucleotide sequence of the listeriolysin O (hlyA) gene (GenBank accession number M24199). The nucleotide sequences of the primer sites are underlined, left and right arrows indicate complementary and sense sequences that are used; (B), diagram exhibiting the positions at which the primers attach to amplify the target sequence.
