Table 1.
Primers | Sequence (5′-3′) | Length |
---|---|---|
F3 | TCAAGTTGTGAATGCAATTTCGA | 23 nt |
B3 | GCTCTTTAGTAACAGCTTTGCCG | 23 nt |
FIP1(F1c-1 + F2-1) | CGTTTTACAGGGAGAACATCTGGTTGttttCTAACCTATCCAGGTGCTCTCG | 52 mer |
FIP2(F1c-2 + F2-2) | GCGTTGTTAACGTTTGATTTAGTGGCttttCACTCAGCATTGATTTGCCAGGT | 53 mer |
BIP2(B1c-1 + B2-1) | TGACGAAATGGCTTACAGTGAATCACttttGCGCCGAAGTTTACATTCAAGCT | 53 mer |
BIP1(B1c-2 + B2-2) | AATCAGTGAAGGGAAAATGCAAGAAGttttCTGGAAGGTCTTGTAGGTTCAT | 52 mer |
LF1 | TCTACTAATTCCGAATTCGCT | 21 nt |
LF2 | CAACGATTTTATTGTCTTGATTAG | 24 nt |
LB2 | TGCGAAATTTGGTACAGCAT | 20 nt |
LB1 | GTCATTAGTTTTAAACAAATTTACTATAACG | 31 nt |
P1 | CTAACCTATCCAGGTGCTCTCG | 22 nt |
P2 | CACTCAGCATTGATTTGCCAGGT | 23 nt |
B2 | GCGCCGAAGTTTACATTCAAGCT | 23 nt |
B1 | CTGGAAGGTCTTGTAGGTTCAT | 22 nt |