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. 2015 Sep 25;20(10):17747–17759. doi: 10.3390/molecules201017747

Figure 3.

Figure 3

Protective effects of P. major hydroethanolic extract in isolated rat liver mitochondria exposed to the oxidant t-BOOH. Mitochondria (1 mg/mL) were incubated with PmHE (0.1 mg/mL) in the presence of 0.6 mM t-BOOH. (A) ROS (reactive oxygen species) generation was accompanied by monitoring DCF (2′,7′-dichlorodihydrofluorescein) fluorescence at 503/529 nm excitation/emission, respectively; traces are representative of (at least) three experiments with different mitochondrial preparations; (B) GSH (glutathione, reduced form) and (C) NAD(P)H levels measured fluorimetrically, as described in Materials and Methods. Data were presented as the mean of three experiments with different mitochondrial preparations; (D) Lipid peroxidation was as TBARS. Percentages were calculated by considering positive control as 100% and data were presented as mean ± S.E.M. of three experiments in triplicate with different mitochondrial preparations. * Statistically different from t-BOOH, and # statistically different from control (p < 0.05).