Table 2.
Glucosinolate (GSL) biosynthesis-related genes in B. oleracea as compared between Bolbase and EnsemblPlants.
| Gene Name | Biosynthesis Pathway | B. rapa | B. oleracea (Bolbase) | Total CDS of Bolbase | B. oleracea (Ensemblplants) | Total CDS of EnsemblPlants | Matching Sequence (%) *** |
|---|---|---|---|---|---|---|---|
| Transcription factors | Aliphatic and indolic GSLs | Bra012961 | Bol007795 (Cun) | 558 | Bo2g161590.1 | 1059 | 554(98.0) |
| MYB28 | Bra035929 | Bol036286 (C09) | 615 | Bo9g014610.1 | 1083 | 476(93.0) | |
| Bra029311 | Bol017019 (C06) | 426 | Bo7g098590.1 ** | 1068 | 426(99.0) | ||
| Bol036743 (C05) | 426 | Bo7g098590.1 ** | 1068 | 426(98.0) | |||
| MYB29 | Bra009245 | Bol008849 (C03) | 513 | Bo3g004500.1 | 993 | 513(94.0) | |
| Bra005949 | |||||||
| MYB34 | Bra013000 | Bol017062 (C06) | 951 | Bo7g098110.1 | 948 | 951(97.0) | |
| Bra035954 | Bol007760 (Cun) | 843 | Bo2g161180.1 | 843 | 843(97.0) | ||
| Bra029349 | Bol036262 (C09) | 294 | Bo9g014380.1 | 882 | 294(100.0) | ||
| Bra029350 | |||||||
| MYB51 | Bra016553 | Bol013207 (C08) | 1002 | Bo8g067910.1 | 1002 | 1002(100.0) | |
| Bra031035 | Bol030761 (C05) | 990 | Bo5g025570.1 | 990 | 990(99.0) | ||
| Bra025666 | |||||||
| MYB122 | Bra015939 | Bol026204 (C07) | 981 | Bo6g118350.1 | 1113 | 981(100.0) | |
| Bra008131 | |||||||
| Dof1.1 | Bra030696 | Bol023400 (C08) | 1005 | Bo8g010700.1 | 1350 | 915(99.0) | |
| Bra031588 | Bol041144 (C05) | 1011 | Bo5g008360.1 | 1011 | 1011(100.0) | ||
| Bol006511 (C08) | 936 | Bo8g112940.1 | 936 | 936(100.0) | |||
| IQD1 | Bra034081 | Bol023096 (C01) | 1656 | Bo1g144340.1 | 1368 | 1260(95.0) | |
| Bra001299 | Bol033935 (Cun) | 1437 | Bo3g061890.1 | 1437 | 1437(99.0) | ||
| TFL2 | Bra023629 | Bol021358 (C02) | 1146 | Bo2g013840.1 | 1152 | 1146(94.0) | |
| Bra013958 | Bol000201 (Cun) ** | 1236 | Bo9g159960.1 ** | 1372 | 1236(99.0) | ||
| Bra006417 | Bol019784 (C09) ** | 1236 | Bo9g159960.1 ** | 1372 | 1236(99.0) | ||
| Bol034455 (C03) | 999 | Bo3g012730.1 | 1248 | 906(95.0) | |||
| BCAT-4 | Aliphatic GSLs | Bra022448 | Bol018130 (C05) | 1083 | Bo5g113720.1 | 1083 | 1083(99.0) |
| Bra001761 | Bol026690 (C03) | 1083 | Bo3g073430.1 | 825 | 825(100.0) | ||
| MAM1/2 | Bra029355 | Bol017071 (C06) | 1236 | Bo7g098000.1 | 1527 | 1243(81.0) | |
| Bra013009 | Bol020647 (C03) | 1518 | Bo2g161100.1 ** | 1518 | 1518(99.0) | ||
| Bra029356 | Bol020646 (C03) | 804 | Bo2g161100.1 ** | 1518 | 760(82.0) | ||
| Bra021947 | Bol037823 (C04) | 1302 | Bo2g102060.1 | 1494 | 880(78.0) | ||
| Bra013011 | Bol017070 (C06) | 1527 | Bo7g098000.1 | 1527 | 1527(99.0) | ||
| MAM3 | Bra018524 | Bol016496 (C02) | 759 | Bo2g102060.1 | 1494 | 759(100.0) | |
| CYP79F1 | Bra026058 | Bol038222 (C05) | 1551 | Bo5g021810.1 | 1623 | 1551(99.0) | |
| CYP83A1 | Bra032734 | Bol040365 (C04) | 1506 | Bo4g130780.1 | 1506 | 1505(99.0) | |
| Bra016908 | Bol005188 (C04) | 570 | Bo4g191120.1 | 1509 | 570(100.0) | ||
| GSTF11 | Bra032010 | Bol000843 (Cun) | 633 | Bo5g150180.1 | 648 | 633(98.0) | |
| GSTU20 | Bra003645 | Bol021558 (C07) | 654 | Bo6g081630.1 | 654 | 654(99.0) | |
| UGT74C1 | Bra021743 | Bol006450 (Cun) | 1371 | Bo4g177530.1 | 657 | 647(99.0) | |
| Bra005641 | Bol014127 (C04) | 1371 | Bo4g049480.1 | 1372 | 1371(98.0) | ||
| STb | Bra015938 | Bol026202 (C07) | 1035 | Bo6g118360.1 | 1035 | 1035(100.0) | |
| Bra015936 | Bol026201 (C07) | 1035 | Bo6g118370.1 | 1035 | 1035(100.0) | ||
| STc | Bra025668 | Bol030757 (C05) | 1014 | Bo5g025610.1 | 1014 | 1014(99.0) | |
| FMOGS-OX2 | Bra027035 | Bol010993 (Cun) | 1386 | Bo9g037180.1 | 1386 | 1386(100.0) | |
| FMOGS-OX5 | Bra016787 | Bol029100 (C08) | 1347 | Bo8g062610.1 | 1347 | 1347(99.0) | |
| Bra026988 | Bol031350 (C08) | 1380 | Bo8g108390.1 | 1380 | 1380(100.0) | ||
| AOP2 | Bra034180 | Bol045938 | ? | Bo9g006240.1 | 1032 | ||
| Bra018521 | Bol045939 | ? | Bo2g102190.1 | 1104 | 1105 **** | ||
| Bra000848 | Bol045940 | ? | Bo3g052110.1 | 948 | 497 **** | ||
| GS-OH | Bra022920 | Bol033373 (C04) ** | 243 | Bo4g173530.1 ** | 1077 | 219(99.0) | |
| Bra021670 | Bol033374 (C04) ** | 243 | Bo4g173530.1 ** | 1077 | 219(99.0) | ||
| CYP79B2 | Indolic GSLs | Bra010644 | Bol032767 (C03) | 1557 | Bo3g152800.1 | 1557 | 1557(100.0) |
| Bra011821 | Bol028852 (C01) | 1623 | Bo1g002970.1 | 1623 | 1623(97.0) | ||
| Bra017871 | Bol018585 (C06) | 1626 | Bo7g118840.1 | 1626 | 1626(100.0) | ||
| CYP79B3 | Bra030246 | Bol031784 (Cun) | 1632 | Bo4g149550.1 | 1632 | 1632(99.0) | |
| CYP83B1 | Bra034941 | Bol033477 (C08) | 1473 | Bo8g024390.1 | 1500 | 1473(100.0) | |
| GSTF9 | Bra021673 | Bol033376 (C04) | 648 | Bo4g173610.1 | 648 | 648(99.0) | |
| Bra022815 | Bol004624 (C03) | 648 | Bo3g024840.1 | 648 | 648(99.0) | ||
| GSTF10 | Bra022816 | Bol004625 (C03) | 648 | Bo3g024850.1 | 648 | 648(99.0) | |
| STa | Bra008132 | Bol039395 (C02) | 1014 | Bo2g080910.1 | 1014 | 1014(100.0) | |
| Bra015935 | Bol026200 (C07) | 1017 | Bo6g118380.1 | 1017 | 1017(100.0) | ||
| CYP81F1 | Bra011762 | Bol028913 (C01) | 1500 | Bo1g003680.1 | 1437 | 882(97.0) | |
| Bra011761 | Bol028914 (C01) | 1497 | Bo1g003710.1 | 1497 | 1497(98.0) | ||
| Bol017375 (C07) | 369 | Bo6g095040.1 ** | 942 | 324(99.0) | |||
| Bol017376 (C07) | 246 | Bo6g095040.1 ** | 942 | 232(97.0) | |||
| CYP81F2 | Bra002747 | Bol012237 (C09) | 933 | Bo9g131960.1 | 1581 | 933(99.0) | |
| Bra020459 | Bol014239 (C02) | 1482 | Bo2g032590.1 | 1482 | 1482(99.0) | ||
| Bra006830 | Bol026044 (C03) | 1482 | Bo3g019420.1 | 1482 | 1482(100.0) | ||
| CYP81F3 | Bra010597 | Bol032711 (C03) | 1491 | Bo3g153480.1 | 1491 | 1491(99.0) | |
| Bra011758 | Bol028919 (C01) | 1500 | Bo1g004740.1 | 1500 | 1500(99.0) | ||
| CYP81F4 | Bra010598 | Bol032712 (C03) | 1506 | Bo01007s020.1 ** | 1506 | 1506(99.0) | |
| Bra011759 | Bol032714 (C03) | 960 | Bo01007s020.1 ** | 1506 | 807(98.0) | ||
| Bol028918 (C01) | 1503 | Bo1g004730.1 | 1506 | 1503(97.0) | |||
| IGMT1 | Bra012270 | Bol007029 (C08) | 1119 | Bo8g070650.1 | 1119 | 1119(100.0) | |
| Bra012269 | Bol020663 (C05) | 342 | Bo09472s010.1 | 312 | 342(100.0) | ||
| IGMT2 | Bra016432 | Bol007030 (C08) | 1125 | Bo8g070660.1 | 1350 | 1120(99.0) | |
| GGP1 * | Both aliphatic and indolic GSLs | Bra024068 | Bol033672 (C06) | 753 | Bo7g114570.1 | 753 | 753(99.0) |
| Bra011201 | Bol018073 (C01) | 753 | Bo1g012070.1 | 753 | 753(100.0) | ||
| Bra010283 | Bol012989 (C03) | 753 | Bo3g175530.1 | 753 | 753(100.0) | ||
| SUR1 * | Bra036490 | Bol038767 (C09) | 1209 | Bo7g003330.1 | 1371 | 1209(99.0) | |
| Bra036703 | Bol038764 (C09) ** | 459 | Bo28705s010.1 ** | 207 | 207(100.0) | ||
| Bol038765 (C09) ** | 459 | Bo28705s010.1 ** | 207 | 207(100.0) | |||
| Bol029775 (Cun) | 1008 | Bo7g003330.1 | 1371 | 991(89.0) | |||
| UGT74B1 * | Bra024634 | Bol005786 (C05) | 1311 | Bo5g041080.1 | 1401 | 1311(99.0) | |
| TGG1 | Breakdown aliphatic and indolic GSLs | Bra039825 | Bol017328 (C07) | 822 | Bo6g095780.1 | 411 | 373(99.0) |
| Bra039824 | |||||||
| Bra039823 | |||||||
| Bra016676 | |||||||
| Bra004012 | |||||||
| TGG2 | Bra036914 | Bol028319 (C08) | 1179 | Bo8g039420.1 | 1638 | 1163(99.0) | |
| Bol025706 (C03) | 663 | Bo2g155820.1 | 714 | 187(98.0) | |||
| TGG4 | |||||||
| TGG5 | Bol031599 (C07) | 1326 | Bo09266s010.1 | 163 | 163(100.0) | ||
| PEN2 | Bra004840 | Bol030092 (C04) | 1299 | Bo4g023800.1 | 3350 | 937(99.0) | |
| Bra004839 |
* Participates in biosynthesis of both aliphatic and indolic GSLs; ** These genes have the same CDS sequence in both databases; *** the number of matching base pairs between the sequence in Bol sequence (http://www.ocri-genomics.org/bolbase/) and that in EnsemblPlants (http://plants.ensembl.org/Brassica_oleracea/Info/Index?db=core); **** comparison between B. rapa and B. oleracea EnsemblPlants sequence.