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. 2015 Jul 7;20(7):12376–12388. doi: 10.3390/molecules200712376

Table 1.

The gradient elution program of the HPLC.

Time (min) A% (Acetonitrile) B% (0.03% Phosphoric Acid)
0 5 95
15 10 90
30 14 86
35 25 75
60 25 75