Table 1.

Antimicrobial activity of methanol extracts of propolis from the Andean slopes (“precordillera”), central valley, and coastal area (“secano costero”), Región del Maule, Chile. Results are presented as MIC values in µg/mL.

Propolis Sample	Microorganisms a
	Gram (+)						Gram (−)
	1	2	3	4	5	6		7	8	9	10	11
Andean slopes
Romeral 1	500	125	31.2	250	62.5	125		125	125	62.5	125	500
Romeral 2	250	125	62.5	250	62.5	125		62.5	125	62.5	250	>1000
Romeral3	500	250	125	250	250	250		250	250	250	250	500
Romeral 4	125	125	62.5	500	125	125		125	125	125	250	>1000
Romeral 5	1000	500	500	500	1000	500		500	250	500	500	>1000
Vilches	125	125	62.5	250	125	250		125	62.5	125	125	250
Central valley
Cumpeo	250	125	31.2	125	62.5	62.5		62.5	62.5	62.5	125	250
San Clemente 1	500	250	125	1000	250	250		250	250	250	250	500
San Clemente 2	125	250	31.2	1000	125	125		125	62.5	62.5	250	500
San Clemente 3	62.5	125	62.5	250	62.5	62.5		62.5	250	62.5	62.5	500
San Clemente 4	62.5	62.5	62.5	1000	125	250		125	62.5	125	125	250
San Clemente 5	500	125	1000	>1000	>1000	1000		500	250	>1000	500	1000
Coastal area
Curepto 1	125	125	125	500	125	250		125	125	125	125	500
Curepto 2	62.5	125	125	500	125	250		250	125	500	500	500
San Javier 1	125	250	62.5	>1000	125	125		250	125	125	250	>1000
San Javier 2	>1000	>1000	62.5	>1000	500	500		1000	500	>1000	500	1000
San Javier 3	500	250	125	250	250	250		250	250	250	250	500
San Javier 4	62.5	125	62.5	1000	125	125		62.5	125	125	250	500
San Javier 5	125	62.5	31.2	250	125	125		62.5	125	62.5	500	500
Cefotaxime	0.5	0.5	0.5	5.0	0.5	0.5		7.5	0.5	12.5	0.5	0.05

^a 1: methicillin-sensitive Staphylococcus aureus ATCC 25923; 2: methicillin-resistant S. aureus ATCC 43300; 3: Escherichia coli ATCC 25922; 4: E. coli 121; 5: E. coli 122; 6: E. coli LM₂; 7: Pseudomonas sp.; 8: Yersinia enterocolítica- PI; 9: Salmonella enteritidis MI; 10: Salmonella sp (LM); 11: Proteus mirabilis 94-2.