Figure 2.

Post-insult mitochondria-targeted antioxidant treatment reduces ischemia/reperfusion-induced brain damage. Animals were subjected to ischemia for 1 h followed by reperfusion for 24 h. The mitochondria-targeted antioxidants were injected i/p immediately after the beginning of reperfusion at a dose of 1 µmol/kg. (A) Representative T2-weighted magnetic resonance (MR) images were obtained 24 h after reperfusion onset (each image covered an 0.8 mm thick brain section). Hyperintense regions in the right hemisphere (shown as more light area) refer to ischemic areas; (B) Infarct volume and (C) brain edema (swelling) evaluated by using magnetic resonance imaging (MRI) with analysis of T2-weighted images; (D) Neurological status estimated using limb-placing test. * denotes significant difference from the MCAO + Vehicle group (p < 0.05) (One-way ANOVA, followed by Tukey’s post hoc analysis for (B,C); Kruskal-Wallis test with the Mann-Whitney u-test for (D).