Figure 1.

Apigenin inhibits proliferation and viability of human melanoma cells and induces anoikis. (A) Cell viability was quantified by MTT assay in attached and detached condition. A2058 and A375 cells were treated with different concentrations of apigenin for 24 h (a and b) and for different time intervals (c and d) at 37 °C; * and ** Indicates significant differences (p < 0.05) compared to the untreated control (B) A375 and A2058 cells were left untreated or were treated with apigenin at different concentrations for 24 h and morphological changes of the cells were observed by light microscopy and photographed at a magnification of 100×.