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. 2015 Nov 27;20(12):21157–21166. doi: 10.3390/molecules201219752

Figure 2.

Figure 2

Inhibitory effect of apigenin on integrin protein expression. (A) mRNA expression of ITGAV, ITGA5, ITGA4 and ITGB3 in human melanoma A2058 and A375 cells. Cells were incubated with various concentrations of apigenin for 18 h. Cells were harvested and ITGAV, ITGA5, ITGA4 and ITGB3 mRNA levels were analyzed by RT-PCR; (B) Protein expression of ITGAV, ITGA5, ITGA4 and ITGB3 in Human melanoma A2058 and A375 cells. Cells were incubated with various concentrations of apigenin for 18 h. Cells were harvested and ITGAV, ITGA5, ITGA4 and ITGB3 protein levels were analyzed by western blotting. RT-PCR and western blotting were performed in triplicate and data are representative of three separate experiments.