Table 2.
Effects of different metal ions on activity of Tricholoma matsutake α-galactosidase a.
| Metal Ion Concentration | Relative—Galactosidase Activity (%) | |||
|---|---|---|---|---|
| 10 mM | 5 mM | 2.5 mM | 1.25 mM | |
| Mg2+ | 30.6 ± 0.03 | 80.90.01 | 82.6 ± 0.02 | 93.0 ± 0.04 |
| Mn2+ | 43.3 ± 0.03 | 69.1 ± 0.07 | 101.6 ± 0.02 | 100.4 ± 0.02 |
| Pb2+ | 9.6 ± 0.02 | 80.0 ± 0.12 | 88.3 ± 0.09 | 95.3 ± 0.05 |
| Al3+ | 72.1 ± 0.01 | 80.7 ± 0.03 | 73.1 ± 0.05 | 82.5 ± 0.02 |
| Cu2+ | ND | ND | 31.8 ± 0.01 | 80.9 ± 0.05 |
| Fe3+ | ND | ND | ND | 31.4 ± 0.02 |
| EDTA | 116.4 ± 0.65 | 96.3 ± 0.84 | 101.3 ± 0.61 | 112.5 ± 0.45 |
a The activity of α-galactosidase without incubation with metal ions was set as 100%. Results represent mean ± SD, n = 3. The detailed data for each metal ion (Mg2+, Mn2+, Pb2+, Al3+, Cu2+ and Fe3+) are shown in Tables S1–S6 in the supplementary file. “ND” means not detected.