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. 2015 Jul 24;20(8):13550–13562. doi: 10.3390/molecules200813550

Table 4.

Kinetic parameters for hydrolysis of pNPGal, raffinose and stachyose by Tricholoma matsutake α-galactosidase.

Substrate Km (Mm) Kcat a (s−1) Kcat/Km (s−1·mM−1)
pPNGal 0.99 9.17 9.3
Raffinose 3.7 6.4 1.73
Stachyose 3.5 6.7 1.9

a Turnover number (Kcat) is defined here as the number of mmoles of product s−1·mmol−1 of α-galactosidase, given by the relationship Vmax/[E].