Table 2.

Solvent gradient program of UPLC analysis.

Time (min)	Flow (mL/min)	A (%)	B (%)
0	0.300	99.0	1.0
1.0	0.300	99.0	1.0
12	0.300	91.0	9.0
17	0.300	88.0	12.0
17.5	0.300	86.5	13.5
23	0.300	86.5	13.5
33	0.300	70	30.0
35	0.300	60	40.0
50	0.300	35	65.0
52	0.300	35	65.0
55	0.300	1.0	99.0