Table 2.
Cytotoxic activities of compounds 1–6.
| µg/mL | HT29 (% of Variation vs. Control, Mean ± SD) | Caco-2 (% of Variation vs. Control, Mean ± SD) | IEC6 (% of Variation vs. Control, Mean ± SD) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 10 | 50 | 100 | 1 | 10 | 50 | 100 | 1 | 10 | 50 | 100 | |
| 2D culture (3 assays) | ||||||||||||
| Acetyl Glucocucurbitacin E (1) | 4.9 ± 0.1 | −7.4 ± 0 | −4.1 ± 0.1 | −0.2 ± 0 | −6.7 ± 0.1 | −45.5 ± 0.1 | −55.4 ± 0 | −53.7 ± 0 | −6.7 ± 0 | 0 ± 0 | 15.5 ± 0 | 23.2 ± 0 |
| Coumaroyl-acetyl Glucocucurbitacin I (2) | −6.4 ± 0.1 | −4.1 ± 0 | −4.7 ± 0.1 | −3.8 ± 0.1 | −47.7 ± 0.1 | −45.2 ± 0.1 | −52.1 ± 0 | −35.8 ± 0 | −23.7 ± 0 | −24.7 ± 0 | −2.2 ± 0 | 7.3 ± 0 |
| Cucurbitacin E (3) | 8.5 ± 0.2 | 4.8 ± 0 | 13.9 ± 0 | 16.4 ± 0 | −11.4 ± 0 | 7.1 ± 0.1 | −8.0 ± 0 | −0.3 ± 0.1 | −1.2 ± 0 | 7.7 ± 0 | 20.9 ± 0 | 27.9 ± 0 |
| Glucocucurbitacin E (4) | 11.4 ± 0.1 | −0.1 ± 0.1 | −9.7 ± 0.2 | 7.5 ± 0.1 | −2.7 ± 0.2 | 11.1 ± 0.1 | 4.4 ± 0 | −1.1 ± 0 | 8.1 ± 0 | 7.2 ± 0 | 36.0 ± 0 | 29.3 ± 0 |
| Cucurbitacin I (5) | 18.8 ± 0.1 | 6.3 ± 0 | 5.8 ± 0.1 | 10.7 ± 0 | −11.0 ± 0.1 | 1.9 ± 0.1 | −3.1 ± 0 | −1.4 ± 0 | −1.8 ± 0 | 8.7 ± 0.1 | 2.2 ± 0.1 | −14.0 ± 0.1 |
| Glucocucurbitacin I (6) | 2.7 ± 0.1 | 2.3 ± 0 | −1.6 ± 0.1 | 2.8 ± 0 | −2.6 ± 0.1 | −45.3 ± 0.1 | −49.1 ± 0 | −55.4 ± 0 | −0.8 ± 0 | 4.0 ± 0 | 10.7 ± 0 | 16.3 ± 0.1 |
| 3D culture (2 assays) | ||||||||||||
| Acetyl Glucocucurbitacin E (1) | 0 ± 0.1 | −24.0 ± 0.2 | −6.2 ± 0.4 | nd | −3.5 ± 0.4 | −3.3 ± 0.1 | −23.0 ± 0.1 | nd | −13.2 ± 0.1 | 2.0 ± 0 | −17.8 ± 0.3 | nd |
| Coumaroyl-acetyl Glucocucurbitacin I (2) | −32.5 ± 0.1 | −31.9 ± 0.1 | −51.7 ± 0.1 | nd | −19.0 ± 0.2 | −10.1 ± 0.1 | −54.0 ± 0.1 | nd | 5.7 ± 0.3 | 2.0 ± 0 | −24.4 ± 0.1 | nd |
| Glucocucurbitacin I (6) | 23.2 ± 0.1 | 1.2 ± 0.3 | 1.8 ± 0.4 | nd | −1.5 ± 0.2 | −1.7 ± 0.4 | −12.1 ± 0.2 | nd | −2.6 ± 0.1 | −5.4 ± 0.1 | −9.1 ± 0.1 | nd |
nd: not determined.