. 2015 Oct 22;20(10):19277–19290. doi: 10.3390/molecules201019277

Table 1.

Bacterial strains, plasmids, and oligonucleotide primers used in this study.

Strain, Plasmid, or Primer	Relevant Characteristics, Description, or Sequence	Source
Bacterial Strains
M. smegmatis ATCC 14468	reference strain	American Type Culture Collection (ATCC)
M. smegmatis mc²155 (ATCC 70084)	reference strain	ATCC
M. tuberculosis TCGH 57612	Clinical strain	Buddhist Tzu Chi General Hospital (BTCGH)
M. tuberculosis TCGH 58339	Clinical strain	BTCGH
M. tuberculosis TCGH 58821	Clinical strain	BTCGH
M. tuberculosis TCGH 59490	Clinical strain	BTCGH
M. tuberculosis TCGH 62046	Clinical strain	BTCGH
M. tuberculosis TCGH 45545	MDR Clinical strain	BTCGH
M. tuberculosis TCGH 17037	XDR Clinical strain	BTCGH
E. coli Top10	Laboratory strain for TA cloning use	Invitrogen, San Diego, CA, USA
E. coli BL21 (DE3)	Laboratory strain for protein expression	Invitrogen
E. coli ATCC 25922	Gram-negative reference strain	ATCC
A. baumannii ATCC 17978	Gram-negative reference strain	ATCC
S. enteric BCRC 10746	Gram-negative reference strain	Bioresource Collection and Research Center (BCRC)
B. subtilis BCRC 10447	Gram-positive reference strain	BCRC
S. aureus ATCC 25923	Gram-positive reference strain	ATCC
Plasmids
pGEM-T-easy	3015-bp E. coli vector, Amp^r, P_lac, lacZ	Promega, San Diego, CA, USA
pET30b	5421-bp E. coli vector, Km^r, P_T7, His-Tag	Novagen, Madison, WI, USA
pGEM-LysA	pGEM-T-easy::1566 bp of lysA	This study
pGEM-LysB	pGEM-T-easy::741 bp of lysB	This study
pET30b-LysA	pET-30b::1566 bp of lysA; His-Tag-LysA under P_T7	This study
pET30b-LysB	pET-30b:: 741 bp of lysB; His-Tag-LysB under P_T7	This study
Primers
BTCU-1_lysA-FP	5ʹ-ATGACGGAACGGGTACTCCC-3ʹ	This study
BTCU-1_lysA-RP	5ʹ-TCACTGCTCGATGACCCTGTT-3ʹ	This study
BTCU-1_lysB-FP	5'-GTGAGCGACCGCTGGCT-3ʹ	This study
BTCU-1_lysB-RP	5ʹ-TCATGTCAAGTCGCGTAGAAACT-3ʹ	This study