Table 1.
In vitro time-of-addition studies on anti-influenza virus activity of CLK1 inhibitors in MDCK cells by CPE reduction assay against H1N1.
| Samples | Name | CC50 a | CC0 b | Pre-Treatment Assay | Simultaneous Treatment Assay | Post-Treatment Assay | |||
|---|---|---|---|---|---|---|---|---|---|
| μg/mL | EC50 (μg/mL)c | SI d | EC50 (μg/mL) | SI | EC50 (μg/mL) | SI | |||
| J10688 | Clypearin | >200 | 100 | 0.75 ± 0.13 | >266.67 | 1.2 ± 0.28 | >166.67 | 18.08 ± 3.02 | >11.06 |
| J12098 | Corilagin | 153.54 | 30 | 3.99 ± 3.72 | 38.48 | 2.00 ± 2.22 | 76.77 | 5.91 ± 1.44 | 25.98 |
| J14848 | Pinosylvin | 18.26 | 10 | 7.54 ± 5.48 | 2.42 | 5.28 ± 2.45 | 3.46 | 6.65 ± 4.3 | 3.23 |
| J12133 | Chebulanin | 90.27 | 30 | 15.29 ± 10.35 | 5.90 | 9.84 ± 0.44 | 9.17 | 21.11 ± 11.15 | 4.28 |
| J25986 | Propyl gallate | 182.00 | 30 | 15.94 ± 6.94 | 11.42 | 12.88 ± 8.06 | 14.13 | 3.42 ± 0.2 | 53.22 |
| J20353 | Hispidulin | 60.35 | 30 | 18.89 ± 5.1 | 3.19 | 5.26 ± 2.48 | 11.47 | >30 | ND |
| J21548 | Norwogonin | 91.79 | 30 | >30 | ND e | >30 | ND | >30 | ND |
| J11534 | - | 175.31 | 30 | >30 | ND e | >30 | ND | >30 | ND |
| J10610 | Kaempferol | 68.50 | 30 | 5.16 ± 2.4 | 13.28 | 12.34 ± 5.89 | 5.55 | 23.3 ± 1.08 | 2.94 |
| J20725 | Luteolin | 24.86 | 10 | >10 | ND | >10 | ND | 5.74 ± 0.54 | 4.33 |
| J10857 | - | 77.72 | 30 | 18.43 ± 12.99 | 4.22 | 16.27 ± 2.29 | 4.78 | 14.67 ± 5.25 | 5.30 |
| J14528 | Isorhapontigenin | 83.37 | 30 | 5.98 ± 2.3 | 13.94 | >30 | ND | >30 | ND |
| J14080 | Epigallocatechin gallate | 71.10 | 30 | 10.19 ± 6.22 | 6.98 | 14.86 ± 1.81 | 4.78 | >30 | ND |
| Methyl gallate | Methyl gallate | 69.50 | 30 | >30 | ND e | >30 | ND | >30 | ND |
| Genistein | Genistein | 75.60 | 30 | >30 | ND | >30 | ND | >30 | ND |
| Ribavirin | Ribavirin | >200 | 100 | 4.36 ± 0.99 | >45.87 | 10.32 ± 2.38 | >19.38 | 13.78 ± 3.47 | >14.51 |
| TG003 | TG003 | 121.50 | 30 | 5.09 ± 0.78 | 23.87 | 15.75 ± 3.49 | 7.71 | 3.56 ± 1.47 | 34.13 |
a CC50: 50% cytotoxic concentration; b CC0: maximal non-cytotoxic concentration; c EC50: 50% effective concentration; d SI: selective index, CC50/EC50; e ND: not determined.