Table 2.

Comparison of the kinase reaction components in our experiment with Harry J.’s.

Kinase Reaction System	Authors’ Experiment	Menegay’s Experiment
recombinant CLK1	2.98 ng in 20 μL	0.5 μg in 50μL
myelin basic protein(MBP)	1 μg in 20 μL	1 μg in 50 μL
MgCl2	10 mM	10 mM
MnCl2	1 mM	2 mM
ATP	1 μM	10 μM ([γ-32P])
reaction temperature	room temperature	room temperature
reaction time	90 min	20 min