Figure 7.

LPS promoted adipogenesis via JAK/STAT and AMPK-dependent cPLA₂ expression. (A) Serum-starved 3T3-L1 cells were pretreated with AACOCF3 (1 μM), AG490 (100 nM), STAT5-I (10 μM), WP1066 (1 μM) or BML-275 (0.1 μM) for 1 h. (B) Or cells were transfected with siRNA of scramble, cPLA₂, JAK2 or AMPK fro 24 h. Then, cells were incubated with 20 μg/mL of LPS 48 h. Or (C) cells were stimulated without or with arachidonic acid (0.1 or 1 μM) or A769662 (1 or 10 μM) for 24 h. At the end of incubation, the adipogenesis was performed with DM-I and DM-II medium. After the process of adipogenesis, the images were captured by microscope. Data are expressed as means ± SEM of at least 3 independent experiments (n≥3). &P < 0.05 or *P < 0.05, as compared with the control group or scramble siRNA alone group. #P < 0.05, as compared with the LPS treated alone.