Figure 2.

Attenuation of H₂O₂-induced ROS generation by baicalein in RT4-D6P2T cells. Cells were pretreated with the indicated concentration of baicalein or 10 mM NAC for 1 h, and then stimulated with or without 1 mM H₂O₂ for 1 h. (A) The cells were incubated with DCF-DA, and DCF fluorescence was measured by flow cytometry. The values represent the means of two independent experiments. (B) After staining with DCF-DA, images were obtained by fluorescence microscopy (original magnification, ×200). These images are representative of at least three independent experiments. Scale bars, 10 μm.