Fig. 4.

Ibrutinib reduces systemic cell activation during ceftriaxone-treated pneumococcal pneumonia. Mice were infected intranasally with S.pneumoniae bacteria and treated 24 h later with ceftriaxone. Concomitant with ceftriaxone and 12 h later, vehicle or ibrutinib was given and mice were sacrificed 48 h after induction of infection. Mice sacrificed at 24 h after inoculation with S. pneumoniae without additional treatment served as control. a total cells, monocytes and polymorphonuclear cells (PMN) in blood depicted as 10⁶cells/mL. b Expression (geometric mean) of CD11b on blood monocytes and PMN. c Concentrations of myeloperoxidase (MPO) and elastase in plasma. d Concentration of lactate dehydrogenase (LDH), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and urea in plasma. Data are represented as mean and SD. *p < 0.05, **p < 0.01 ((Kruskal-Wallis 1-way analysis of variance test followed by Mann-Whitney U-test). Data represent results from a single experiment