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. 2019 Jan 14;19:52. doi: 10.1186/s12879-018-3601-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cytokine levels in culture supernatant of healthy and leprosy patients. Cytokine levels in culture supernatant of unstimulated (A) M. leprae antigens stimulated (B) PBMCs of healthy individuals (H), TT/BT and BL/LL patients. PBMCs from the study subjects were cultured with or without antigens for 5 days in RPMI at 37 °C in 5% humidified atmosphere. Supernatants were collected after 48 h and 5 days of culture and cytokine was estimated. Concentration of cytokine was normalized after subtracting values of unstimulated supernatant from stimulated supernatant. I = IFNγ, II = IL-4, III = IL-10. * = p < 0.05, ** = p < 0.001. MLSA- M. leprae soluble antigen, WCL- whole cell lysate, PGL-1- Phenolic glycolipid-1. BT/TT (Borderline tuberculoid/Tuberculoid), BL/LL (Borderline lepromatous/Lepromatous)