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. 2019 Jan 14;19:52. doi: 10.1186/s12879-018-3601-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — IFN-γ and IL-4 expressing CD4⁺T cells in healthy individuals and leprosy patients. I Representative histogram showing CD4⁺T cells expressing IFN-γ and IL-4 in healthy individuals and leprosy patients. II Mean Percentage of IFN-γ and III IL-4 expressing CD4⁺T cells in healthy individuals (H) and leprosy patients with or without stimulation with M. leprae antigens. PBMCs of different study subjects were incubated with medium only (A) or stimulated with MLSA, WCL and PGL-1antigens (B) and incubated for 24 h. After incubation cells were washed and stained with fluorescent labeled anti-CD3, anti-CD4, anti IFN-γ and anti-IL-4 antibodies and acquired in flow cytometer. *p < 0.05 and **p < 0.001 *** = p < 0.0001. BT/TT (Borderline tuberculoid/Tuberculoid), BL/LL (Borderline lepromatous/Lepromatous)