Fig. 2.

MDMX and MDM2 knockdown in MDA-MB-231 orthotopic transplants does not significantly reduce primary tumor growth. a Primary tumor volumes of 231.mir30.vector (n = 6), 231.shmdm2 (n = 7), and 231.shmdmx (n = 7) engrafted animals were measured using calipers over 36 days. b The endpoint tumor volumes were determined on dissected masses at the time of necropsy. c mRNA levels of mdm2 and mdmx normalized to gapdh in primary tumors were determined by real-time qRT-PCR. Error bars represent SD. * p < 0.05, ** p < 0.01, *** p < 0.001, NS = nonsignificant. The p value was calculated using two-tailed unpaired t tests. d Protein expression of MDM2, MDMX, and mtp53 from 231.mir30.vector, 231.shmdm2, and 231.shmdmx engrafted primary tumors were determined by Western blot analysis. Three tumors per group were used, and actin is shown as a loading control