Fig. 4.

miR-144 inhibits CCNB1 expression by binding to CCNB1 3′-UTR CCNB1. a Diagram of the putative binding sites of miR-144 on the 3′-UTRs of CCNB1 and the mutated sequence of 3′ UTRs of CCNB1. b The WT 3′-UTR or mutated 3′-UTR of CCNB1 was fused to the luciferase coding region and co-transfected into HEK293T cells with miR-144 mimics. Relative luciferase activity was determined 48 h after transfection. Data are expressed as mean ± SD from 3 independent experiments. **p < 0.01 vs. Vector Control. c RT-PCR analysis of the effect of CCNB1 expression in HepG2 and SMMC-7721 cells after transfection with miR-144 mimics. GAPDH expression levels were detected as an endogenous control. Data are expressed as mean ± SD. Experiments were performed in triplicate and repeated at least three times. **p < 0.01 vs. control. d Western blot analysis of the CCNB1 expression in HepG2 and SMMC-7721 cells after transfection with miR-144 mimics or miR-144 inhibitor. Actin expression levels were detected as an endogenous control. Experiments were repeated twice and representative data was shown