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. 2019 Jan 14;19:16. doi: 10.1186/s12935-019-0733-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Histological evaluation of HNSCC-derived tumor spheroids. a Immunohistochemical staining of HNSCC tumor spheroids with the proliferation marker Ki67 cultured for 1 and 7 days; scale bar = 300 µm. b Quantification of Ki67-positive cells in 1 day and 7 days old tumor spheroids; the data are depicted as mean of ± SD, n = 10. *p < 0.05 according to Student’s test