Fig. 3.

Western blot analysis for HNSCC-derived tumor spheroids grown in 2D and 3D. a Expression of EMT and CSC markers in HNSCC cells grown in 2D and cells grown in 3D. Cells cultured for 1, 3 and 7 day in 3D conditions were subjected for analysis. b Expression of EGFR in HNSCC tumor cells and corresponding 3D cultures collected after 1, 3 and 7 days in culture. Representative blot images and the values of the relative signals obtained by probing with selected antibody normalized to GAPDH are shown