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. 2019 Jan 14;19:24. doi: 10.1186/s12870-019-1628-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cytosine methylation frequency in the 35S promoter of the S₁ progenies. a-c Methylation frequency of plus-strand of the CaMV 35S promoter in S1:345, S1:208 ORN and the control 16c plants. N is the number of the clones used for the bisulfite sequencing. The x-axis is the position relative to the nucleotide distance from the transcription start sites. Bars under the x-axis in b and c are the nucleotide position of the CaMV 35S promoter sequence integrated in the CMV-A1 vectors. d Summary of the results (a to c) to show differences in CHH, CHG, CG and total methylation. The asterisks indicate a statistical significance in methylation frequencies between S1:345 and S1:208 ORN by two-tailed Fisher’s exact test (** P < 0.01)