Fig. 6.

Difference in specific cytosine methylation between S2:208 RED and S2:208 ORN in the Vein tissues. a Difference in methylation frequencies in the overall plus- and minus-strand of the 35S promoter in S2:208 RED and S2:208 ORN. To make it easier to find any differences in methylation frequencies in each strand, values of S2:208 RED Vein (Fig. 5a and c, upper graphs) were subtracted from those of S2:208 ORN Vein (Fig. 5a and c, lower graphs). b Close-up of the subdomain A1 in a. The x-axis is the nucleotide sequence of the subdomain A1. Nucleotide sequences of the plus- and minus-strand of the subdomain A1 are indicated below the graph. Methylated CG and CHG sites are underlined. The asterisks in (b) indicate a statistical significance between S2:208 RED and S2:208 ORN by two-tailed Fisher’s exact test (* P < 0.05)