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. 2019 Jan 14;19:24. doi: 10.1186/s12870-019-1628-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Effect of specific cytosine methylation in the 35S promoter on promoter activity. a Decrease of promoter activity of the 35S sequence by specific cytosine methylation in both strands. b Effect of strand-specific methylation of the 35S promoter on the Fluc expression. c Effect of the methylation of the cytosine residues (positions − 84, − 56 and − 33) at CHH context on the Fluc expression. Fluc activity was calibrated by Rluc activity. Values in the graph are shown as a fold change of the 35S-Fluc control (set to 1.0). Values are means with standard deviations obtained from three biological replicates. Data were analyzed using Tukey-Kramer test. Different lowercase letters at error bars indicate a significant difference between the means at the 5% level