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. 2019 Jan 15;38:18. doi: 10.1186/s13046-018-1011-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — ANKHD1 is a co-regulator with SMYD3 and is associated with poor prognosis of HCC. a Lysates of huh7-SMYD3 and huh7-control cells were immunoprecipitated for endogenous H3K4me3 or ANKHD1 and immunoblotted for ANKHD1 or H3K4me3, respectively. b Subcellular fractions were isolated to analyze the coexpression of SMYD3, ANKHD1 and H3K4me3 in cytoplasm and nucleus using western blot. c Confocal analysis of HCCLM3 and huh7 cells transfected with SMYD3 displaying ANKHD1 (green), H3K4me3 (red) and DAPI (blue) staining; MERGE shows the overlapped images. d IHC analysis of SMYD3 expression in HCC tissues. e Kaplan-Meier analysis of the overall survival or recurrence-free survival in HCC patients according to ANKHD1 expression