Table 1.
Year | Technology | Reference |
---|---|---|
1963 | IVF using in vitro capacitated spermatozoa | 2 |
1972 | Interspecific IVF using zona‐free oocytes (hamster test) | 4 |
1976 | ICSI using epididymal spermatozoa | 12 |
1988 | Development of hamster embryo culture medium lacking glucose and phosphate | 20 |
1992 | Birth of live pups following IVF | 24 |
1993 | Fertilization of oocytes with round spermatids | 29 |
1999 | Vitrification of embryos | 47 |
2002 | Birth of pups following ICSI | 26 |
2004 | Birth of pups following ROSI | 32 |
2014 | Gene targeting by CRISPR/Cas9 | 40 |