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. 2018 Oct 7;18(1):34–39. doi: 10.1002/rmb2.12241

Table 1.

Development of assisted reproductive technologies in golden hamsters

Year Technology Reference
1963 IVF using in vitro capacitated spermatozoa 2
1972 Interspecific IVF using zona‐free oocytes (hamster test) 4
1976 ICSI using epididymal spermatozoa 12
1988 Development of hamster embryo culture medium lacking glucose and phosphate 20
1992 Birth of live pups following IVF 24
1993 Fertilization of oocytes with round spermatids 29
1999 Vitrification of embryos 47
2002 Birth of pups following ICSI 26
2004 Birth of pups following ROSI 32
2014 Gene targeting by CRISPR/Cas9 40