Table 2.
Antibody information
| Target | Catalogue number | Size (kDa) | Dilution (WB) | Dilution (IHC) | Company | Address |
|---|---|---|---|---|---|---|
| GAPDH | sc‐47724 | 36 | 1:2000 | Santa Cruz Biotechnology | Santa Cruz, TX, USA | |
| SIRT1 | 07‐131 | 110 | 1:2000 | EMD Millipore | North Ryde, NSW, Australia | |
| PGC‐1α | NBP1‐04676 | 91 | 1:2000 | Novus Biologicals | Littleton, CO, USA | |
| SREBP1 | 04‐469 | 62 | 1:500 | EMD Millipore | NSW, Australia | |
| GLUT2 | 07‐1402 | 50 | 1:1000 | EMD Millipore | NSW, Australia | |
| p‐Akt (S473) | 13038 | 60 | 1:1000 | Cell Signaling | Danvers, MA, USA | |
| Akt | 9272 | 60 | 1:1000 | Cell Signaling | MA, USA | |
| p‐AMPKα (T172) | 2535 | 62 | 1:1000 | Cell Signaling | MA, USA | |
| AMPKα | 5831 | 62 | 1:1000 | Cell Signaling | MA, USA | |
| Collagen I | ab34710 | 1:750 | Abcam | Cambridge, UK | ||
| Collagen III | ab7778 | 1:750 | Abcam | Cambridge, UK |
WB, western blot.