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Expression of maternal effect genes (MEGs; Mater, Npm2, and Hsf1) in pronuclear embryos, and zygotic gene activation genes (ZGA; Hspa1a, Myc, and Hdac1) in 2‐cell embryos. ICSI embryos were produced by sonication of hamster spermatozoa in TCM199 or TBS + EGTA. Analyses were performed using primer sets Mater, Npm2, and Hsf1 at 6 h post‐ICSI, and Hspa1a, Myc, and Hdac1 at 44 h post‐ICSI. *P < 0.05, Student's t test
