. 2018 Oct 29;18(1):83–90. doi: 10.1002/rmb2.12253

Table 1.

Quantitative PCR primer sequences

Gene		Primers (5′‐3′)	Product size (bp)	Annealing temperature (°C)
Mater	Forward	CCGGCTTACAAAAGCTCAAG	128	60
Mater	Reverse	GACTTGGTTTTTGCGATGGT	128	60
Npm2	Forward	CAGCCCTCAGGACAAGAGTC	108	60
Npm2	Reverse	CCCTCGGACACAGAGATACC	108	60
Hsf1	Forward	TCTCACTGGTGCAGTCGAAC	154	60
Hsf1	Reverse	GGAGACGGAGCTGAGTATGG	154	60
Hspa1a	Forward	CGACCTGAACAAGAGCATCA	213	60
Hspa1a	Reverse	AAGATCTGCGTCTGCTTGGT	213	60
Myc	Forward	CGACTCCACAGCCTTCTCTC	179	60
Myc	Reverse	GCTGCCTCTTTTCCACAGAC	179	60
Hdac1	Forward	CGCATGACTCACAATTTGCT	134	60
Hdac1	Reverse	CGAATGGAACGCAAGAATTT	134	60
18 s	Forward	ATGGCCGTTCTTAGTTGGTG	217	60
18 s	Reverse	CGCTGAGCCAGTCAGTGTAG	217	60