. 2018 Oct 29;18(1):83–90. doi: 10.1002/rmb2.12253

Table 2.

Chromosome analysis of hamster spermatozoa that were frozen‐stored; separated by sonication in TCM199, TBS + EGTA, or TBS + Ca; and then injected into mouse oocytes

Medium used for storage and sonication	No. of zygotes analyzed	No. (%) of oocytes with normal sperm chromosomes	No. of spermatozoa with chromosome aberrations
Medium used for storage and sonication	No. of zygotes analyzed	No. (%) of oocytes with normal sperm chromosomes	Structural^*	Aneuploidy
TCM199	58	40 (69.0)^b	18	0
TBS + EGTA	58	52 (89.7)^a	6	0
TBS + Ca	50	34 (68.0)^b	16	0

TBS + EGTA, 100 mM Tris‐HCl–buffered solution +50 mM EGTA; TBS + Ca, 100 mM Tris‐HCl–buffered solution +2 mM CaCl₂.

Significantly different values (P < 0.05) between different superscripts. Percentages of oocytes with normal sperm chromosomes were analyzed by Fisher's exact probability test and chi‐square test.

Structural chromosome aberrations included chromosome/chromatid‐type breaks and exchanges.