. 2018 Oct 29;18(1):83–90. doi: 10.1002/rmb2.12253

Table 3.

In vitro development of hamster oocytes injected with sperm heads separated by sonication in TCM199 or TBS + EGTA

Medium used for storage and sonication	No. of oocytes injected (no. of replicates)	No. of oocytes with 2PN (%)	No. (%) of embryos developed to
Medium used for storage and sonication	No. of oocytes injected (no. of replicates)	No. of oocytes with 2PN (%)	≥2‐cell	≥8‐cell	≥Morulae	Blastocysts
TCM199	81 (4)	75 (92.6)^a	72 (88.9)^a	53 (65.4)^a	35 (43.2)^a	14 (17.3)^a
TBS + EGTA	88 (4)	80 (90.9)^a	80 (90.9)^a	74 (84.1)^b	69 (78.4)^b	37 (42.0)^b

TBS + EGTA, 100 mM Tris‐HCl–buffered solution +50 mM EGTA; 2PN, two well‐developed pronuclei.

Significantly different values (P < 0.05) between different superscripts within the same column. Percentage of embryo development was analyzed by chi‐square test.