Table 2.
Primer sequences of mouse genes assayed by quantitative real-time PCR.
| Primer | Base sequence | Tm (°C) | Transcription product (bp) |
|---|---|---|---|
| β-Actin-F | ACCCTAAGGCCAACCGTGAAA | 62 | 193 |
| β-Actin-R | ATGGCGTGAGGGAGAGCATA | 61 | |
| IL-17A-F | TCAGCGTGTCCAAACACTGAG | 60.81 | 79 |
| IL-17A-R | CGCCAAGGGAGTTAAAGACTT | 58.22 | |
| IL-17F-F | TGCTACTGTTGATGTTGGGAC | 58.22 | 165 |
| IL-17F-R | CAGAAATGCCCTGGTTTTGGT | 59.31 | |
| IL-22-F | ATGAGTTTTTCCCTTATGGGGAC | 58.39 | 124 |
| IL-22-R | GCTGGAAGTTGGACACCTCAA | 60.48 | |
| IL-1β-F | GAAATGCCACCTTTTGACAGTG | 58.62 | 116 |
| IL-1β-R | TGGATGCTCTCATCAGGACAG | 59.24 | |
| TNF-α-F | TCAAACCCTGGTATGAGCCC | 59.38 | 154 |
| TNF-α-R | ACCCATTCCCTTCACAGAGC | 59.67 |